Role of leucocytes in liver damage in experimental models of type 1 and 2 diabetes mellitus

Diabetes mellitus is a metabolic disorder, which results from insufficient secretion of insulin and/or its action, thus leading to hyperglycemia. Liver damage known be among the most common complications type 2 diabetes (T2D) and in T1D. Comparison leukocyte phenotypes liver tissue with appropriate blood parameters may assess degree search for approaches correction destruction mellitus. Therefore, we aimed assessment changes injury markers numbers leucocytes (CD45 + cells), T lymphocytes (CD3 cells) macrophages experimental models types 1 2diabetes. The experiment was conducted on 30 male Wistar rats. Alloxan at dose 170 mg/kg body weight used T1D modeling. To provide model T2D, streptozotocin nicotinamide were injected doses 65 mg/kg, 110 respectively. Intact animals as comparison control. Biochemical, hematological, immunohistochemical morphometrical methods study. In T2D groups, levels glucose (10.88±0.47 mmol/l 10.78±0.42 mmol/l) glycosylated hemoglobin (6.73±0.78% 6.60±0.20% correspondingly) rather close each other exceeded values intact rats (5.20±0.40 4.07±0.30%). At same time, increase total leucocyte number fraction peripheral against normal more pronounced group than group. both diabetic increased sinusoidal cells, macrophages, CD45 cells CD3+ relative detected. However, group, distributed, mainly, parenchyma, whereas they showed location. similar increasing macrophage numbers, number, higher counts CD3 located parenchyma perivascular area, found T2DM compared this parameter T1DM An ALT activity confirms significant whereas, an AST less indicate uniformly distributed cytolysis. our study showed, that, despite hyperglycemia level, inflammatory process level whole organism local are A severity corresponds cell infiltration tissue.